Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Rockland Immunochemicals 10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) - MB-008
Phosphate buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.
Rockland Immunochemicals 10X SDS-PAGE Running Gel Buffer (0.25 M Tris, 1.92 M Glycine, 1.0% SDS pH 8.3) - MB-017
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. 10X SDS-PAGE Running Buffer is suitable for laboratory involved in protein biochemistry. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
Rockland Immunochemicals 10X TE pH 8.0 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED - MB-007
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
Rockland Immunochemicals 1.0 M Tris HCl pH 7.6 - MB-003
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Rockland Immunochemicals 10X TTBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride 0.1% (v/v) Tween-20) - MB-013
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M Tris HCl, 1.5 M Sodium Chloride and 0.1% (v/v) Tween-20 at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
Boca Scientific Inc BuccalFix DNA/RNA Stabilization Buffer, 25ml, Sufficient for Stabilizing 50 swabs
BuccalFix Stabilization Buffer is designed to completely stabilize the DNA and RNA on buccal swabs by inhibiting all enzymatic and microbial activity that occurs following buccal sampling. DNA can be stored in BuccalFix at ambient temperature for over 2 years, while RNA can be stabilized for 4 days. While stabilized, the structure and integrity of the DNA and RNA is fully maintained for further downstream processing applications. Stabilized DNA in BuccalFix can be processed for isolation by the addition of Proteinase K and subsequent isolation steps.
Medchemexpress LLC Pipes dipotassium | 108321-27-3 | MFCD00069751 | 98.0% | 378.55 | C8H16K2N2O6S2 | 100 G
PIPES (dipotassium) is the dipotassium salt of the zwitterionic buffering agent PIPES, provided as a white to off-white solid. It offers stable buffering near physiological pH with low metal ion binding, making it suitable for biochemical and cell biology applications such as assay buffers, electrophoresis, and sample preparation.
![Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-A22500-100.0.JPG-250.jpg)
Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams
Zwitterionic buffer used in biochemistry and molecular biology research.
- pKa (25°C): 6.88
- The working pH range in aqueous solution is 6.1 - 7. 5
- Absorbance @ 280nm (5% water): <0.02
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 1 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-1000.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 1 Kilograms
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
Research Products International Corp Bicine Ultra-Pure, 250 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
AG Scientific Inc PBS Tablets 100 Tablets
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.